The fully automatic hybridization instrument is a high-throughput and high-efficiency nucleic acid molecular hybridization device suitable for a variety of reverse dot hybridization experiments (such as genotyping, gene mutation detection, pathogen detection and other fields). Fully automatic and standardization of nucleic acid molecular hybridization is realized, and the total time of nucleic acid molecular hybridization experiments is greatly shortened, which can save manpower and achieve technical quality control.